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Annals of Clinical & Laboratory Science 36:103-104 (2006)
© 2006 Association of Clinical Scientists

Letter to the Editor

Reference Intervals of Serum Soluble APO-1 (Fas/CD95) Concentrations in Healthy Adults

Jong Weon Choi
Department of Laboratory Medicine, College of Medicine, Inha University, Incheon, South Korea

Address correspondence to Jong Weon Choi, M.D., Ph.D., Department of Laboratory Medicine, Inha University Hospital, 7-206, 3-ga, Shinheung-dong, Jung-gu, Incheon, 400-711, South Korea; tel 82 32 890 2503; fax 82 32 890 2529; e-mail: jwchoi{at}inha.ac.kr.

To the Editor:

Apoptosis is characterized by a series of morphological and biochemical changes involving the nucleus, cytoplasm, and cell membrane. Several methods have been developed to detect apoptosis on the basis of such changes [1]. An enzyme immunoassay to quantify a soluble form of APO-1 (sAPO-1) was recently introduced, and the biological importance of sAPO-1 is being extensively studied [2].

In a recent study ( Ann Clin Lab Sci 2005;35: 290–296[Abstract/Free Full Text] ), we reported that sAPO-1 has significant relationships to serum lipid levels and body adiposity in healthy adults [3]. In that article, we listed the mean concentrations of serum sAPO-1 in men and women. However, by mistake, the sAPO-1 concentrations read from the standard curve were reported without adjustment for the dilution factor (x 10). In this letter, we correct this error and provide additional data concerning serum sAPO-1 levels in healthy adults (Tables 1Go and 2Go).


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  		Table 1. Reference intervals of serum soluble APO-1 concentrations in healthy Korean men and women.
 

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  		Table 2. Serum sAPO-1 levels in relation to age and gender in healthy Korean adults. The data are means ± SD and [medians].
 
To the author’s knowledge, reference intervals of serum sAPO-1 levels in a large population of healthy subjects have not been previously reported. The manufacturer of sAPO-1 assay kits (human sAPO-1/Fas BMS245 kit; Bender MedSystems, Vienna, Austria) provides the range of serum sAPO-1 levels obtained from only 8 healthy blood donors. In our study, the reference intervals of serum sAPO-1 concentrations were tested in 223 healthy adults (115 men and 108 women; age 25 to 69 yr) using sAPO-1/Fas BMS245 kits (Bender MedSystems). A nonparametric method was used to calculate the reference ranges for serum sAPO-1 (95% confidence intervals).

As shown in Table 1Go, the reference intervals of sAPO-1 were 234 to 853 pg/ml in men and 218 to 872 pg/ml in women, with median levels of 398 and 373 pg/ml, respectively. The intra-assay CVs were 4.5 – 6.8%, based on 20 replicate assays of 3 samples (mean sAPO-1 levels from 291 to 807 pg/ml); the inter-assay CVs were 3.9 – 6.2%, calculated from duplicate results in 10 consecutive assays. The corrected data for serum sAPO-1 concentrations in relation to age and gender, which were multiplied by the dilution factor, are listed in Table 2Go.

A deficiency or an excess of apoptosis is an integral component of a wide variety of diseases. Sensitivity of detection methods to apoptotic cells has differed among published studies. For instance, the apoptotic rates in control subjects show 4- or 6-fold differences according to the detection methods employed [4]. Thus, the apoptotic activity should be compared only within studies that use the same technique.

Taking these findings into consideration, our data for sAPO-1 in healthy subjects have clinical utility, since these data can be used as reference values for interpreting the results of serum sAPO-1 concentrations in patients with various diseases.

References

  1. Lassus P, Hibner U. Detection and quantification of apoptosis in transiently transfected adherent cells. Nucleic Acids Res 1998;26:5233–5234.[Abstract/Free Full Text]
  2. Kamihira S, Yamada Y. Soluble Fas (APO-1/CD95) isoform in adult T-cell leukemia. Leuk Lymphoma 2001;41:169–176.[Medline]
  3. Choi JW, Kim SK. Relationships of soluble APO-1 (Fas/CD95) concentrations, obesity, and serum lipid parameters in healthy adults. Ann Clin Lab Sci 2005;35:290–296.[Abstract/Free Full Text]
  4. Choi JW. Apoptotic rate varies with detection methods in myelodysplastic syndromes: impact of method selection. Eur J Haematol 2002;69:62–63.[Medline]




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