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Annals of Clinical and Laboratory Science, Vol 8, Issue 2, 106-110
Copyright © 1978 by Association of Clinical Scientists

Articles

The measurement of total serum proteins by the Biuret method

MM Lubran

The biuret reaction for proteins provides a simple and precise method for measuring serum proteins; Beer's law is obeyed to at least 10 g per dl. Several stable biuret reagents are available. Hemoglobin is the only important cause of interference which cannot be minimized by use of a sample blank. The mechanism of the biuret reaction is described and attention is drawn to the heterogeneity of the serum proteins and to the use of a certified albumin standard.




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Copyright © 1978 by the Association of Clinical Scientists.