Annals of Clinical and Laboratory Science, Vol 7, Issue 6, 469-478
Copyright © 1977 by Association of Clinical Scientists
Simple, direct determination of serum 5'-nucleotidase
ES Baginski,
SS Marie,
E Epstein,
and
B Zak
A simple determination of 5'-Nucleotidase in blood serum without deproteinization is described. The enzyme activity is distinguished from that of a nonspecific alkaline phosphatase by nickel inhibition and the inorganic phosphate released from adenosine monophosphate used as substrate is determined by a method previously described. Additional studies include the determination of optimal conditions for the reaction and nickel inhibition.