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Annals of Clinical and Laboratory Science, Vol 6, Issue 4, 312-317
Copyright © 1976 by Association of Clinical Scientists

Articles

Monitoring the administration of methotrexate in antimetabolite therapy

E Epstein, ES Baginski, and B Zac

An enzymatic method for the measurement of methotrexate (MTX) in serum is presented in which the inhibition of the enzyme dihydrofolate reductase by MTX is measured. Reduction of the substrate dihydrofolate by the enzyme and cofactor NADPH is lessened in direct proportion to the amount of MTX present. Measurements can be made in the "therapeutic range" of MTX which corresponds to the 10(-7) to 10(-8) M concentration of MTX in serum.




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Copyright © 1976 by the Association of Clinical Scientists.