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Annals of Clinical & Laboratory Science 40:135-143 (2010)
© 2010 Association of Clinical Scientists

Green Tea (Camelia sinensis) Suppresses B Cell Production of IgE Without Inducing Apoptosis

Ehab Hassanain1,*, Jonathan I. Silverberg1,2,*, Kevin B. Norowitz3, Seto Chice1, Martin H. Bluth5, Neil Brody4, Rauno Joks2, Helen G. Durkin2 and Tamar A. Smith-Norowitz3
Departments of 1 Pathology, 2 Medicine, 3 Pediatrics, and 4 Dermatology, State University of New York Downstate Medical Center, Brooklyn, New York, and 5 Deptartment of Pathology, Wayne State University School of Medicine, Detroit, Michigan

Address correspondence to Tamar A. Smith-Norowitz, Ph.D., Dept of Pediatrics, Box 49, SUNY Downstate Medical Ctr., 450 Clarkson Ave., Brooklyn, NY 11203, USA; tel 718 270 1295; fax 718 270 3289; e-mail tamar.smith-norowitz{at}downstate.edu.

Green tea (Camelia sinensis) is known to possess biological properties that are antioxidative and antimutagenic. Recent studies demonstrated beneficial effects of green tea in inflammatory allergy. However, the effect of green tea on anti-allergic activity/IgE responses in vitro has not been studied. U266 myeloma cells (2 x 106/ml), which secrete IgE, were cultured for 0–72 hr with or without green tea extract (1–300 ng/ml), and IgE levels in the supernatants were determined (24–72 hr) by ELISA. The effects of green tea extract on U266 cell numbers, viability, and apoptosis were studied by flow cytometry. High levels of IgE produced by U266 cells were observed at 24, 48, and 72 hr (1.3 ± 0.3 x 103, 1.7 ± 0.3 x 103, 2.8 ± 0.4 x 103 IU/ml, respectively). Addition of green tea extract either as (a) a single dose, or (b) repeated daily doses, suppressed IgE production with increasing suppression over time (up to 90%; p <0.05); the suppression was dose-dependent with the highest concentrations resulting in the greatest suppression. The suppression of IgE production by green tea extract was not mediated by apoptosis or cell death. This study demonstrates that green tea extract has immunoregulatory effects on human IgE responses in vitro.

Keywords: IgE, U266 myeloma cells, B cell, polyphenols, flow cytometry, annexin, propidium iodide






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