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Annals of Clinical and Laboratory Science, Vol 4, Issue 4, 250-254
Copyright © 1974 by Association of Clinical Scientists

Articles

Quantitation of A2 hemoglobin by polyacrylamide gel disc electrophoresis: a method with individual specimen standardization

HG Schriever and DM Leveckis

A polyacrylamide gel electrophoresis technic for the determination of hemoglobin A2 is presented. Rapid separation is an advantage. The use of a diluted hemolysate as a 4 percent standard avoids overestimation of the A2 fraction by densitometry and also provides a reference for visual comparison. Normal range of A2 hemoglobin by this method is 1.12 to 4.13 per dkg. Coefficient of variation was 5.84 percent.




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Copyright © 1974 by the Association of Clinical Scientists.