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Annals of Clinical & Laboratory Science 39:409-412 (2009)
© 2009 Association of Clinical Scientists

Case Report

PCR Detection of Histoplasma capsulatum var. capsulatum in Whole Blood of a Renal Transplant Patient with Disseminated Histoplasmosis

Julianne Qualtieri1, Charles W. Stratton1,2, David R. Head1 and Yi-Wei Tang1,2
Departments of 1 Pathology and 2 Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee

Address correspondence to Yi-Wei Tang, M.D., Ph.D., Molecular Infectious Disease Laboratory, Vanderbilt University Hospital, 4605 TVC, Nashville, TN 37232-5310, USA; tel 615 322 0126; fax 615 343 8420; e-mail yiwei.tang{at}vanderbilt.edu.

We report the identification of Histoplasma capsulatum var. capsulatum from whole blood in a renal transplant patient with disseminated histoplasmosis using colorimetric microtiter-plate PCR. This modality demonstrated utility in reaching a definitive diagnosis in a timely manner. Blood fungal cultures in this case remained negative, suggesting that molecular assays may facilitate the laboratory diagnosis of disseminated histoplasmosis.

Keywords: Histoplasma capsulatum, histoplasmosis, PCR-EIA






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