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Annals of Clinical & Laboratory Science 39:134-137 (2009)
© 2009 Association of Clinical Scientists

Brief Communication

Immunotargeting of Apolipoprotein E in Amyloid: An Initial Trial in Mice

Toshiyuki Yamada1, Tomonosuke Someya2 and Shinobu Fujita3
1 Department of Clinical Laboratory Medicine, Jichi Medical University, Tochigi,2 Department of Pediatrics, Juntendo University School of Medicine, Tokyo, and3 Mitsubishikasei Institute, Tokyo, Japan

Address correspondence to Toshiyuki Yamada, M.D., Department of Clinical Laboratory Medicine, Jichi Medical University, 3311 Yakushiji, Shimotsuke, Tochigi 329-0498, Japan; tel 0285 58 7386; fax 0285 44 9947; e-mail toshiyam{at}jichi.ac.jp.

Apolipoprotein E is commonly present in systemic amyloid deposits. To investigate the possibility of using apolipoprotein E immunotargeting in the diagnosis and treatment of amyloidosis, we examined whether anti-apolipoprotein E monoclonal antibody was bound to murine amyloid deposits in vivo and whether it influenced amyloidogenesis. This study utilized a radiolabeled monoclonal antibody specific to human apolipoprotein E fragments and human apolipoprotein E-knock-in mice, in which AA amyloidosis was induced. Accumulation of the injected radiolabeled antibody was significantly higher in the organs of amyloidotic mice than in those of non-amyloidotic mice. Plasma clearance of the antibody did not differ between the amyloidotic and non-amyloidotic mice. The antibody was given to mice during amyloid induction but failed to prevent amyloidogenesis. The results of this initial study are encouraging, but considerable improvement is necessary, particularly in regard to development of a high affinity antibody.

Keywords: amyloid, apolipoprotein E, immunotargeting, reactive amyloidosis






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