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Annals of Clinical & Laboratory Science 38:247-253 (2008)
© 2008 Association of Clinical Scientists

Variables that Affect Platelet Function Analyzer-100 (PFA-100) Closure Times and Establishment of Reference Intervals in Korean Adults

Young-Uk Cho, Seongsoo Jang, Chan-Jeoung Park and Hyun-Sook Chi
Department of Laboratory Medicine, University of Ulsan, College of Medicine, and Asan Medical Center, Seoul, Korea

Address correspondence to Hyun-Sook Chi, M.D., Ph.D., Department of Laboratory Medicine, Asan Medical Center, 388-1 Poongnap-dong, Songpa-gu, Seoul, Korea; tel 82 02 3 010 4502; fax 82 02 478 0884; e-mail hschi{at}amc.seoul.kr.

The PFA-100 is an in vitro system for measurement of platelet function in whole blood. To establish reference intervals in the Korean population, we investigated the relationships between PFA-100 closure times and gender, ABO blood group, citrate concentration, and time of blood collection in 120 well-characterized healthy Korean adults. Gender did not affect closure time values. Blood group O was associated with longer collagen/epinephrine (CEPI) and collagen/ADP (CADP) closure times than non-O groups (p <0.0001 for both). Closure times were shorter in samples obtained in the morning vs the afternoon. Regression analysis showed an association between CEPI and CADP closure times (p <0.0001), but there were no associations between PFA-100 results and WBC count, hemoglobin, hematocrit, platelet count, and platelet-associated parameters. The reference intervals for CEPI and CADP closure times of 3.2% citrated blood were 80–162 and 64–121 sec, respectively. The reference intervals for PFA-100 closure times in healthy Korean adults were similar to those in Western populations. Flow obstruction was observed in 31 of 480 (6.5%) measurements, but routine performance of duplicate measurements was otherwise unnecessary because of acceptable CV values. PFA-100 closure time should be interpreted with consideration of the patient’s clinical presentation and factors such as ABO blood group and diurnal variation.

Keywords: PFA-100, healthy Korean adults, reference intervals, platelet function test






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