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Annals of Clinical & Laboratory Science 38:41-46 (2008)
© 2008 Association of Clinical Scientists

Comparison of the Seeplex Reverse Transcription PCR Assay with the R-mix Viral Culture and Immunofluorescence Techniques for Detection of Eight Respiratory Viruses

Kyoung Ho Roh1, Jeeyong Kim1, Myung-Hyun Nam1, Sooyung Yoon1, Chang Kyu Lee1, Kapno Lee1, Young Yoo2, Min Ja Kim3 and Yunjung Cho1
1 Departments of Laboratory Medicine, 2 Pediatrics, and 3 Internal Medicine, Korea University College of Medicine, Seoul, Korea

Address correspondence to Dr. Yunjung Cho, Department of Laboratory Medicine, Korea University Medical College, 126-1 Anam-dong, Sungbuk-gu, Seoul, Korea 136-705; tel 82 2 920 5612; fax 82 2 920 5538; e-mail eqcho1ku{at}korea.ac.kr.

This study evaluated the clinical usefulness of a newly introduced multiplex reverse transcription PCR assay (Seeplex RV; Seegene, Inc., Seoul, Korea) in patients with respiratory symptoms. Fifty clinical respiratory specimens (45 from children, 5 from adults) were tested for 8 viruses (influenza virus type A and B, parainfluenza virus type 1, 2, 3, respiratory syncytial virus type A and B, and adenovirus) by Seeplex RV (S-RV) and R-mix viral culture with immunofluorescence (VC-IF). Forty (80%) of the 50 samples showed concordant results between S-RV and VC-IF; 24 of these showed the same positive and 16 showed the same negative results. Among the 10 discrepant samples, 9 were S-RV-positive and VC-IF-negative. Six were obtained in patients with lower respiratory tract infection. Only 1 sample was VC-IF-positive and S-RV-negative. This patient had pneumonia. In 3 cases, more than 1 virus was identified by S-RV. The total running time of S-RV was 6 hr, which shortens the detection time for the viral presence by 2 workdays compared to VC-IF. In conclusion, S-RV is reliable, rapid, relatively easy to perform, and able to detect more than 1 virus simultaneously. Therefore, implementation of the S-RV assay in clinical laboratories will aid rapid diagnosis and treatment of major viral infections of the respiratory tract.

Keywords: respiratory viruses, reverse transcription PCR, virus culture, Seeplex RV assay




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