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Annals of Clinical & Laboratory Science 37:330-334 (2007)
© 2007 Association of Clinical Scientists

A Chromogenic Substrate Culture Plate for Early Identification of Vibrio vulnificus and Isolation of Other Marine Vibrios

Yukari Nakashima1, Megumi Oho1, Kouji Kusaba1, Zenzo Nagasawa1, Osamu Komatsu2, Isao Manome2, Kazukuni Araki3, Hirotaka Oishi4 and Mikio Nakashima3
1 Department of Clinical Laboratory Medicine, Saga University Hospital, Saga; 2 Biochemical Research Laboratory, Eiken Chemical Co., Ltd., Tokyo; 3 Department of Anesthesiology and Critical Care Medicine, Saga University Faculty of Medicine, Saga; 4 Ariake Sea Research Project, Saga University, Saga, Japan

Address correspondence to Hirotaka Oishi, M.D., Ph.D., Ariake Sea Research Project, Saga University, 5-1-1, Nabeshima, Saga City, Saga, 849-8501, Japan; tel 81 952 34 2373; fax 81 952 34 2056; e-mail ooishih{at}cc.saga-u.ac.jp.

Vibrio vulnificus infection can result in necrotizing fasciitis and sepsis, which have short latentcy periods and high mortality rates. Thus, an easy and quick detection method is needed to improve the outcome. To distinguish V. vulnificus from other pathogens that cause necrotizing fasciitis, we developed a selective isolation culture agar plate (Chromochecker Vibrio Agar-1; CVA-1) for use in environmental monitoring and in the clinical setting. One hundred four strains of V. vulnificus, already identified biochemically, showed typical colony form and color when grown on CVA-1. Thirty-six of 51 marine bacteria samples suspected to be V. vulnificus on CVA-1 were subsequently identified as V. vulnificus by a biochemical identification system. Of 8 bacteria known to cause necrotizing fasciitis, only V. vulnificus grew on CVA-1. In addition, growth on CVA-1 allowed ready differentiation of Vibrio species. CVA-1 can be used to distinguish pathogenic Vibrios according to colony form and chromatic differences.

Keywords: Vibrio vulnificus, chromogenic substrate culture plate, necrotizing fasciitis, sepsis






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