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Annals of Clinical & Laboratory Science 37:256-259 (2007)
© 2007 Association of Clinical Scientists

Comparison of the Abbott Architect i2000 Assay, the Roche Modular Analytics E170 Assay, and an Immunoradiometric Assay for Serum Hepatitis B Virus Markers

Hyunjung Kim1, Eun-Jee Oh1, Mi-Sook Kang1, Sung Hoon Kim2 and Yeon-Joon Park1
1 Departments of Laboratory Medicine and 2 Radiology, College of Medicine, The Catholic University of Korea, Seoul, Korea

Address correspondence to Yeon-Joon Park, M.D., Department of Laboratory Medicine, The Catholic University of Korea, Kangnam St. Mary’s Hospital, 505 Banpo-dong, Seocho-ku, Seoul, 137-701, South Korea; tel 82 2 590 1604; fax 82 2 592 4190; e-mail yjpk{at}catholic.ac.kr.

Serum hepatitis B virus (HBV) markers are the most important data for epidemiological screening and clinical diagnosis of HBV infection, especially in endemic areas. We compared the results of the Roche Modular Analytics E170 assay, the Abbott Architect i2000 assay, and an immunoradiometric assay (IRMA) for HBV surface antigen (HBsAg), anti-HBV surface antigen (anti-HBs), HBV e antigen (HBeAg), and anti-HBV e antigen (anti-HBe). A number of serum samples (264, 263, 224, and 202 for HBsAg, anti-HBs, HBeAg, and anti-HBe, respectively) were studied. For samples giving discrepant results for HBeAg between methods, real-time PCR assays were performed. The concordance rates among the three methods were high for HBsAg (100%) and HBeAg (94.6), but low for anti-HBs (91.6%) and anti-HBe (82.2%). For anti-HBs, which could be measured quantitatively by the Modular E170 and Architect i2000 procedures, discrepant results were observed at low levels of anti-HBs. For anti-HBe, the positive rate was highest with Modular E170 (60.9%) followed by the IRMA kit (54.1%) and Architect i2000 (51.0%). This study shows substantial differences between the assay results by the three methods, which should be taken into account in determinations of serum HBV markers.

Keywords: automated analyzers, hepatitis B viral markers, immunoradiometric assay






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