ACLS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hidaka, H.
Right arrow Articles by Katsuyama, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hidaka, H.
Right arrow Articles by Katsuyama, T.
Annals of Clinical & Laboratory Science 37:213-221 (2007)
© 2007 Association of Clinical Scientists

Analysis of Human Serum Lipoprotein Lipid Composition Using MALDI-TOF Mass Spectrometry

Hiroya Hidaka1, Noboru Hanyu2, Mitsutoshi Sugano3, Kenji Kawasaki3, Kazuyoshi Yamauchi3 and Tsutomu Katsuyama4
1 Department of Biomedical Laboratory Science, School of Health Sciences, Shinshu University, Matsumoto; 2 Clinical Laboratories, Shinonoi General Hospital, Shinonoi; 3 Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto; and 4 Department of Laboratory Medicine, Shinshu University School of Medicine, Matsumoto, Nagano Prefecture, Japan

Address correspondence to Hiroya Hidaka, Ph.D., Department of Biomedical Laboratory Science, School of Health Sciences, Shinshu University, Asahi 3-1-1, Matsumoto, Nagano, 390-8621, Japan; tel 81 263 37 2800; fax 81 263 34 5316; e-mail hiroyan{at}hsp.md.shinshu-u.ac.jp.

This study used matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify all lipid classes in human serum lipoproteins. After the major lipoproteins classes were isolated from serum by ultracentrifugation, the lipids were extracted and mixed with 2,5-dihydroxybenzoic acid (2,5-DHB) dissolved in Folch’s solution (chloroform/methanol 2:1, v/v). MALDI-TOF MS analysis of the samples identified phospholipids (PLs), lysophospholipids (lysoPLs), sphingolipids (SLs), triglycerides (TGs), cholesteryl esters (CEs), and free cholesterol; it also showed the characteristics of individual fatty acid chains in serum lipids. MALDI-TOF MS allowed analysis of strongly hydrophobic and non-polar molecules such as CEs and TGs as well as hydrophilic molecules such as phospholipids. Direct analysis of fatty acids was not possible. The concentrations of lipids were not consistent with the ion peak intensities, since the extent of polarity affected the ionization characteristics of the molecules. However, lipid molecules with similar molecular structures but various fatty acid chains, such as phosphatidylcholine (PCs), were analyzed quantitatively by MALDI-TOF MS. Quantitative measurement of cholesterol was possible with the use of an internal standard. This study shows that MALDI-TOF MS can be used for direct investigation and quantitative analysis of the phospholipid composition of serum lipoproteins.

Keywords: MALDI-TOF mass spectrometry, lipoproteins, lipids, phospholipids, lysophospholipids






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2007 by the Association of Clinical Scientists.