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Annals of Clinical & Laboratory Science 36:307-311 (2006)
© 2006 Association of Clinical Scientists

Effect of Sodium Hydroxybutyrate on the Expression of Hippocampal N-Methyl-D-Aspartate Receptor 2B Subunit mRNA in Neonatal Rats with Hypoxic-Ischemic Insult

Zheng-Liang Ma1, Xiao-Ping Gu1, Yin-Ming Zeng2 and Yan Zhang2
1 Department of Anesthesiology, Drum Tower Hospital, Medical Department of Nanjing University, Nanjing; and 2 Affiliated Hospital of Xuzhou Medical School, Anesthesia Institute of Jiangsu Province, People’s Republic of China

Address correspondence to Dr. Zehng Liang Ma, Department of Anesthesiology, Drum Tower Hospital, Medical Department of Nanjing University, Nanjing 210008, People’s Republic of China; tel 86 25 8330 4616; fax 86 25 8331 7016; e-mail mazhengliang1964{at}yahoo.com.cn.

The objective of this research was to test whether sodium hydroxybutyrate (GHB-Na) protects rat neonatal brain against hypoxia-ischemia (HI). Specifically, the objective was to determine the effect of GHB-Na administration on the expression of N-methyl-D-aspartate subunit (NR2B) mRNA in the rat hippocampus. Seven-day-old Sprague-Dawley rats were subjected to ligation of the left carotid artery and were randomly assigned to 5 groups: sham operated (S), saline treated (C), and those treated with GHB-Na (G1, G2, G3), at 3 dosages (50, 100, or 200 mg/kg, ip, thrice daily). NR2B mRNA levels in the left hippocampus were assayed at 2, 6, 12, 24, 72, and 168 hr after HI (exposure to 8% O2/92% N atmosphere for 2 hr). The results suggest that HI insult increased NR2B mRNA gene expression in the left hippocampus of the neonatal rats and that GHB-Na administration partially suppressed this effect of HI insult.

Keywords: rat brain, hypoxia, ischemia, carotid ligation, hydroxybutyrate, hippocampus, N-methyl-D-aspartate receptors, mRNA






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