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Fatty Acid Synthase and its mRNA Concentrations Are Decreased at Different Times Following Hoechst 33342-induced Apoptosis in BC3H-1 Myocytes

Address correspondence to Frederick L. Kiechle, M.D., Ph.D., 18811 Riverside Drive, Beverly Hills, MI 48025, USA; tel 248 646 2724; fax 248 551 3694; e-mail fkiechle{at}hotmail.com.

Fatty acid synthase (FAS) regulates the production of fatty acids and plays a role in regulating apoptosis. Hoechst 33342-induced apoptosis in BC3H-1 myocytes was used as a model to explore intracellular changes in FAS protein (Western blot) and FAS mRNA (RT-PCR). Total lipid and individual phospholipid synthesis was inhibited by a lethal dose of Hoechst 33342 (20 µg/ml) while total lipid and phospholipid degradation ([1-¹⁴C]-acetate pulse chase method) were not. Hoechst 33342 at 20 µg/ml reduced the concentration of FAS protein, which was followed more than 6 hr later by a reduction in FAS mRNA. In conclusion, the inhibition of fatty acid synthesis induced by 20 µg/ml of Hoechst 33342 is attributed to the degradation of FAS protein by activated caspases rather than by inhibition of FAS enzyme activity or FAS mRNA synthesis.

Keywords: Hoechst 33342, apoptosis, BC3H-1 myocytes, fatty acid synthase, RT-PCR