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Annals of Clinical & Laboratory Science 36:79-87 (2006)
© 2006 Association of Clinical Scientists

Correlation of the Turbo-MP RIA with ImmunoCAP FEIA for Determination of Food Allergen-Specific Immunoglobulin E

Kris J. Kontis1, Andre Valcour2, Ashok Patel1, Andy Chen1, Jan Wang1, Julia Chow1 and Narayan Nayak1
1 Hycor, Garden Grove, California; 2 Laboratory Corporation of America, Burlington, North Carolina

Address correspondence to Kris J. Kontis, Ph.D., Hycor Stratagene, 7272 Chapman Ave., Garden Grove, CA 92841, USA; tel 714 933 3148; fax 714 891 4153; e-mail kkontis{at}hycorbiomedical.com.

It has been reported that in vitro measurement of food-specific IgE can be used to accurately predict food allergy and reduce the risk associated with double-blinded placebo-controlled food challenges (DBPCFC). Our objective was to assess the performance characteristics of the Hycor Turbo-MP quantitative radioimmunoassay for food-specific IgE and to determine this method’s comparability to another assay, the Pharmacia ImmunoCAP fluorescence enzyme immunoassay (FEIA). The dynamic range of the Turbo-MP assay is 0.05 to 100 IU/ml, compared to 0.35 to 100 IU/ml for the FEIA. Performance characteristics of the Turbo-MP assay (ie, reproducibility of the calibration curve, within-run precision, total precision, parallelism, and linearity) were determined using samples from the Hycor serum bank. The precision (CV) of IgE calibrator replicates was <10%. The total precision (CV) of the Turbo-MP assay ranged from 8.8% to 18.4% for specific IgE concentrations between 0.28 to 31.4 IU/ml. Testing of serial dilutions of sera with IgE specificities for egg white, cow’s milk, codfish, wheat, peanut, and soybean showed that the assay is linear over the entire dynamic range. Serial dilution data (slopes of 1.01 to 1.10) showed parallelism to serial dilutions of the IgE calibrator (slope of 0.96). The Turbo-MP and FEIA methods were both used for quantitative assays of food-specific IgE in 457 serum samples obtained from a clinical reference laboratory. Comparison of specific IgE results by the Turbo-MP and FEIA methods for 6 major food allergens exhibited a slope of 0.99 (0.92 to 1.03) with a correlation coefficient of 0.81.

Keywords: immunoglobulin E, specific IgE assay, radioimmunoassay, food allergy






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