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Annals of Clinical & Laboratory Science 36:7-15 (2006)
© 2006 Association of Clinical Scientists

Intracellular IL-4, IL-10, and IFN-{gamma} Levels of Leukemic Cells and Bone Marrow T Cells in Acute Leukemia

Hun Hee Park1, Myungshin Kim2, Bong-Hee Lee1, Jihyang Lim2, Yonggoo Kim, Eun Jung Lee2, Woo Sung Min3, Chang Suk Kang2, Won Il Kim2, Sang In Shim2 and Kyungja Han2
1 School of Life Science and Biotechnology, Korea University; 2 Department of Clinical Pathology and 3 Department of Internal Medicine, College of Medicine, Catholic University of Korea, Seoul, Korea

Address correspondence to Kyungja Han, M.D., Department of Clinical Pathology, Catholic University Medical College, St. Mary’s Hospital, Youngdeungpogu, Youido-dong 62, Seoul, Korea (South) 150-713; tel 82 2 3779-1297; fax 82 2 783 6648; e-mail hankja{at}catholic.ac.kr.

The quantitative levels of intracellular cytokines IL-4, IL-10, and IFN-{gamma} (ie, the number of bound PE-conjugated antibody molecules/cell) of leukemic cells and bone marrow T cells (bmT cells) of acute leukemia patients were analyzed by flow cytometry. One hundred, thirty-one (95 AML, 25 ALL, 11 ABL) patients were studied. The leukemic cell IL-4 level was highest in the monocytic AML group (1735 ± 1056) and lowest in the dysplastic AML group (960 ± 545). The IFN-{gamma} level was highest in the acute promyelocytic leukemia (APL) group (495 ± 159), and lowest in the ALL group (252 ± 119). The IL-10 level was not significantly different among the diagnosis groups. In bmT cells, the IL-10 level was highest in the dysplastic AML group (972 ± 1049) and lowest in the APL group (397 ± 352). The leukemic cell cytokine levels were lowest and bmT cell cytokine levels were highest in the dysplastic AML group. There were no significant correlations of these cytokine levels with 2-yr survival rate, complete remission (CR) rate, or relapse rate. The cytokine levels of bmT cells at the time of CR became normal and were not different among the diagnosis groups. In summary, leukemic cell and bmT cell cytoplasmic expression profiles of IL-4, IL-10, and IFN-{gamma} are characteristic for each diagnostic group of acute leukemia patients and the profiles of bmT cells are normal at the time of CR.

Keywords: IL-4, IL-10, IFN-{gamma}, acute leukemia, T lymphocyte, bone marrow T cells






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