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Annals of Clinical & Laboratory Science 36:39-46 (2006)
© 2006 Association of Clinical Scientists

Betamethasone Affects Cerebral Expressions of NF-{kappa}B and Cytokines that Correlate with Pain Behavior in a Rat Model of Neuropathy

Weiying Xie1, Saizhen Luo2, Haojun Xuan2, Chiuwen Chou1, Ganlin Song4, Ran Lv1, Yi Jin1, Wei Li1 and Jianguo Xu1
1 Department of Anesthesiology, Jinling Hospital, Medical School of Nanjing University, Nanjing;2 College of Science, Nanchang University; 3 Department of Cardiothoracic Surgery, Tongde Hospital, Hangzhou, and 4 Art and Design College, Nanchang University, Nanchang, People’s Republic of China

Address correspondence to Jianguo Xu, Ph.D., Department of Anesthesiology, Jinling Hospital, 305 East Zhongshan Road, Nanjing 210002, P.R.China; tel 86 25 8480 6839; fax: 86 25 8480 6839; e-mail: xwyxieweiying{at}hotmail.com.

The objective of this study was to investigate whether corticosteroids modulate neuropathic pain by altering cerebral expression of nuclear factor-kappa B (NF-{kappa}B) and specific cytokines. The effects of topical betamethasone on neuropathic pain and cerebral expression of NF-{kappa}B and cytokines were studied in a rat model of L5 spinal nerve transaction. Behavioral testing was undertaken on days 1, 3, 7, 14, and 21 post-operation using the von Frey and Hargreaves tests. NF-{kappa}B activation in the brain was investigated by an electrophoretic mobility shift assay (EMSA), and cerebral expressions of tumor necrosis factor-{alpha} (TNF{alpha}), interleukin-1ß (IL-1ß), and interleukin-10 (IL-10) were quantified using enzyme-linked immunosorbent assays (ELISA). Spinal nerve transection induced mechanical allodynia and thermal hyperalgesia, which were significantly ameliorated by topical injection of betamethasone around the site of injury. In addition, betamethasone reduced the activation of NF-{kappa}B and elevation of TNF{alpha} and IL-1ß, and induced the expression of IL-10 in the brain, all of which correlated with the changes of pain thresholds in rats. The results suggest that topical betamethasone injection inhibits the development and maintenance of neuropathic pain. Betamethasone may act by regulating the expression of NF-{kappa}B, TNF{alpha}, IL-1ß and IL-10 in the brain. This study yields new insight into the mechanisms of corticosteroid action in neuropathic pain and may provide a basis for clinical pain control.

Keywords: betamethasone, neuropathic pain, hyperalgesia, allodynia, NF-{kappa}B, TNF{alpha}, IL-1ß, IL-10




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