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Annals of Clinical & Laboratory Science 35:455-458 (2005)
© 2005 Association of Clinical Scientists

Technical Note

Preservation of Clinical Isolates of Mycobacterium tuberculosis Complex Directly from MGIT Culture Tubes

Tsi-Shu Huang1,2, Yao-Shen Chen1,3, Susan Shin-Jung Lee1, Hui-Zin Tu1 and Yung-Ching Liu1,4
1 Section of Microbiology and Infectious Diseases, Kaohsiung Veterans General Hospital; 2 Department of Nursing, Yuh-Ing Junior College of Health Care and Management; 3 Graduate Institute of Environmental Education, National Kaohsiung Normal University; 4 Department of Internal Medicine, National Yang-Ming University; Kaohsiung, Taiwan, Republic of China

Address correspondence to Yung-Ching Liu, M.D., Section of Infectious Diseases, Kaohsiung Veterans General Hospital, 386 Ta-Chung 1st Rd, Kaohsiung, Taiwan, R.O.C.; tel 886 07 346 8098; fax 886 07 346 8296; e-mail tshuang{at}isca.vghks.gov.tw.

Preservation of M. tuberculosis complex strains isolated from clinical specimens is important for epidemiological investigations related to tuberculosis. In this study the efficacy of preservation was evaluated by calculating the recovery rate of preserved strains, with various patterns of resistance, after periods of storage and subculture. The recovery rates from strains preserved in enriched solid medium were >90% for storage periods ≤6 yr. However, this procedure for storing mycobacteria is time-consuming, labor-intensive, and impractical for routine use in a clinical laboratory setting. This study shows that recovery rates for strains preserved directly from MGIT fluids are satisfactory for storage periods ≤2 yr. No significant difference in viability was observed within 3 categories of drug resistance: (i) all-susceptible, (ii) multi-drug resistant (MDR), and (iii) a combination of other patterns of resistance. Preserving clinical M. tuberculosis strains directly from MGIT culture fluid fits easily into laboratory routine and is feasible for use in a clinical laboratory setting.

Keywords: Mycobacterium tuberculosis, mycobacterial culture and preservation, antibiotic resistance




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