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Annals of Clinical & Laboratory Science 35:323-328 (2005)
© 2005 Association of Clinical Scientists

Bile Acid Biosynthesis during Liver Regeneration: Enzyme Activities of Cholesterol 7{alpha}-Hydroxylase and 3ß-Hydroxy-{Delta}5-C27-Steroid Dehydrogenase in Rats

Yorio Maeda, Hidehito Nagatomo, Naoya Kuroki, Junji Nagatomo, Fujio Uchiyama, Koji Shimozono, Yoshiaki Kohno and Kazuo Chijiiwa
Department of Surgery 1, Miyazaki Medical College, Kiyotake, Miyazaki, Japan.

Address correspondence to Yorio Maeda, M.D., Department of Surgery 1; Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-1601, Japan; tel 81 985 85 2808; fax 81 985 85 5814; e-mail maedayo{at}post.miyazaki-med.ac.jp.

The effects of two-thirds partial hepatectomy on bile acid metabolism have not been well established. This study investigated the changes in microsomal enzymes activities during liver regeneration. The cholesterol 7{alpha}-hydroxylase (7{alpha}-hydroxylase) and 3ß-hydroxy-{Delta}5-C27-steroid dehydrogenase (3ß-dehydrogenase) activities in male Wistar rats were determined using high-performance liquid chromatography (HPLC). Cytochrome P450 (P450) and cytochrome b5 (b5) levels and NADPH cytochrome c reductase activities were also determined after hepatectomy. 7{alpha}-Hydroxylase activities were not reduced on days 3, 5, or 7 compared to those of sham-operated rats, but there was a significant decrease (by 60%) of 3ß-dehydrogenase activity compared to that of sham-operated groups (p <0.01) on days 3, 5, and 7 after hepatectomy. While 7{alpha}-hydroxylase activity had recovered by day 3 after hepatectomy, 3ß-dehydrogenase activity had not, even on day 7. These results suggest that the mechanisms of regulation of these 2 bile acid metabolizing enzymes are significantly different during liver regeneration.

Keywords: liver regeneration, cholesterol 7{alpha}-hydroxylase, 3ß-hydroxy-{Delta}5-C27-steroid dehydrogenase, cytochrome P450, cytochrome b5, NADPH cytochrome c reductase






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