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Annals of Clinical & Laboratory Science 35:7-14 (2005)
© 2005 Association of Clinical Scientists

Configuration of a New Bioartificial Liver Support System and In Vitro Evaluation of its Functions

Zhong Chen1,2, Yitao Ding3 and Genxi Li1
1 National Key Lab of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing,2 Department of General Surgery, Affiliated Hospital, Nantong University, Nantong,3 Department of Hepatobiliary Surgery, Affiliated Drum Tower Hospital, Medical School, Nanjing University, Nanjing, Jiangsu Province, People’s Republic of China

Address correspondence to Zhong Chen, Ph.D., National Key Lab of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, 210093, Nanjing People’s Republic of China; tel 86 513 5052351; fax 86 513 5106369; e-mail zchen9999{at}sina.com.cn.

The aim of this study was to configure a new bioartificial liver (BAL) support system and evaluate its functions in vitro. Chinese experimental miniature pig hepatocytes were isolated by an in situ recirculating collagenase perfusion method and were cultured in serum-free medium with restriction attachment and spinner technique to form hepatocyte spheroid suspensions containing 1.0 x 1010 hepatocytes. The BAL support system was configured by inoculating the hepatocyte spheroids into the cell circuit of a hollow fiber bioreactor (BIOLIV A3A, Cell Biotech Limited, HK, China). The number and viability of hepatocytes, the levels of alanine aminotransferase (ALT), total bilirubin (TBi), and albumin (ALB) in the circulating hepatocyte suspension and RPMI-1640 medium, and lidocaine metabolism were determined during 6 hr of circulation in the BAL devices. Independent experiments were performed 5 times. There were no significant changes in the number and viability of the hepatocytes during the circulation period. The BAL support system demonstrated substantial albumin synthesis and lidocaine metabolism. The results indicate that the new BAL support system has the ability to perform liver functions and could be used to treat liver failure or provide temporary liver support in patients who are candidates for liver transplantation.

Keywords: bioartificial liver, porcine hepatocyte culture, liver transplantation, lidocaine metabolism






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