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Preventing PCR Amplification Carryover Contamination in a Clinical Laboratory

Address correspondence to Jaber Aslanzadeh, Ph.D., Department of Pathology and Laboratory Medicine, Hartford Hospital, 80 Seymour Street, Hartford, CT 06102, USA; tel 860 545 4128; fax 860 545 2726; e-mail jaslanz{at}harthosp.org.

Abstract

During the past two decades PCR and several other DNA/RNA amplification techniques have become important diagnostic tools in clinical laboratories. Amplification products contamination has been the main impediment to using these techniques routinely in diagnostic laboratories. Over the years, several creative pre- and post-amplification methods have been developed that prevent amplicon carryover contamination. These procedures, coupled with automated systems that employ real-time amplification and simultaneous detection in a closed system, have substantially reduced the possibility of false positive results due to amplification products carryover contamination.

Keywords: polymerase chain reaction, isopsoralen, uracil N-glycosylase, amplicon carryover contamination