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Annals of Clinical & Laboratory Science 34:57-62 (2004)
© 2004 Association of Clinical Scientists

Oxidative Stress and Enzymatic Antioxidant Status in Patients with Nonalcoholic Steatohepatitis

Mehmet Koruk1, Seyithan Taysi2, M. Cemil Savas1, Omer Yilmaz3, Fatih Akcay2 and Metin Karakok4
1 Departments of Gastroenterology and 4 Pathology, Gaziantep University Medical School, Gaziantep, and
2 Departments of Biochemistry and 3 Gastroenterology, Atatürk University Medical School, Erzurum, Turkey

Address correspondence to Mehmet Koruk, M.D., Gazi Mah. 8 Nolu Sk., Enes Apt. 2/5, Gaziantep, Turkey; tel 90 342 339 1313; fax: 90 342 360 3002; e-mail: koruk{at}gantep.edu.tr.

Oxidative stress is an important pathophysiological mechanism in nonalcoholic steatohepatitis (NASH). To assess whether there are relationships between oxidative stress and antioxidant enzymes in the development of NASH, we investigated oxidative stress by measuring serum malondialdehyde (MDA) and nitric oxide (NO) and antioxidant status by measuring serum glutathione (GSH), glutathione peroxidase (GSH-Px), glutathione reductase (GR), and superoxide dismutase (SOD). The study included 18 patients (13 men, 5 women; mean age 42 yr) with biopsy proven NASH and 16 healthy volunteers (10 men, 6 women; mean age 38 yr). Serum levels of MDA, NO, GSH, GSH-Px, GR and SOD were determined by spectrophotometric methods. Serum levels (mean±SD) of MDA (6.7±1.6 vs 2.8±1.7 nmol/ml, p 0.0001), NO (135±28 vs 113±35 mmol/L, p 0.04), GSH (919±137 vs 770±128 mmol/L, p 0.003) were increased in patients with NASH vs controls. Serum levels of GSH-Px (1063±152 vs 1000±94 U/L) and GR (47±22 vs 40±21 U/L) were not singnificantly different in the patients vs controls. However, the serum level of SOD (1.24±0.32 vs 1.51±0.37 U/ml, p: 0.04) was significantly decreased. Impaired antioxidant defense mechanisms may be an important factor in the pathogenesis of NASH. Treatment approaches that affect the antioxidant enzymes may be beneficial in patients with NASH.

Keywords: nonalcoholic steatohepatitis, malondialdehyde, nitric oxide, antioxidant enzymes






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