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Annals of Clinical & Laboratory Science 33:226-231 (2003)
© 2003 Association of Clinical Scientists

Effects of Resveratrol on Lymphocyte Proliferation and Cytokine Release

Paolo Boscolo1, Antonella del Signore2, Enrico Sabbioni3, Mario Di Gioacchino1, Luca Di Giampaolo1, Marcella Reale4, Pio Conti4, Roberto Paganelli1 and Mario Giaccio2
1 Department of Medicine and Science of Aging, 2 Department of Sciences, and 3 Department of Oncology and Neurosciences, University G. D’Annunzio, Chieti and Pescara, Italy; 4 European Commission, Institute for Health and Consumer Protection, ECVAM Unit, Ispra, Italy.

Address correspondence to Professor Paolo Boscolo, Section of Occupational Medicine, Allergy, and Clinical Immunology, University G. D’Annunzio, Faculty of Medicine and Surgery, via dei Vestini, 66013 Chieti Scalo, Italy; tel and fax 39 087 135 6704; e-mail boscolo{at}unich.it.

Resveratrol, synthesized in dietary plants and contained in wine, has been reported to play a beneficial role in certain cardiovascular regulatory mechanisms and to inhibit carcinogenesis by activating immune and inflammatory responses and apoptosis. The object of this study was to elucidate the "in vitro" effects of different concentrations of resveratol (10-4, 10-5, and 10-7 M) on human peripheral blood mononuclear cell (PBMC) proliferation and cytokine release. Spontaneous PBMC proliferation was unaffected by resvetratrol, while the compound at 10-4 M inhibited (69 %) the PHA-stimulated PBMC proliferation. The proliferation stimulation index (ie, the ratio of PHA-stimulated PBMC proliferation/spontaneous PBMC proliferation) of cultures containing 10-4 M resveratrol was very low in relation to the control, while the proliferation stimulation index values at 10-5 and 10-7 M were similar and slightly higher (without statistical significance), respectively. At 10-4 M, resveratrol strongly inhibited PHA-stimulated IFN-{gamma} and TNF-{alpha} release from PBMC, but it did not cause inhibition at 10-5 or 10-7 M. The concomitant immune effects of resveratrol on PBMC proliferation and release of IFN-{gamma} and TNF-{alpha} may be explained by an inhibitory effect on transcription factor NF-kappaB. This study suggests that resveratrol, which is typically present in red wine at about 10-5 M, is unlikely to cause inhibitory immune effects. However, a stimulatory effect of low concentrations of resveratrol on the immune system cannot be excluded.

Keywords: resveratrol, lymphocytes, macrophages, IFN-{gamma}, TNF-{alpha}, immunotoxicity






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