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Annals of Clinical & Laboratory Science 32:399-403 (2002)
© 2002 Association of Clinical Scientists

Immobilization-Induced Changes in Erythrocyte Membrane Fluidity in Rabbits: a Spin-Label Electron Spin Resonance Study

Ming Ju Liu1, Jing Xian Li2, Ling Qin1, Kwong Man Lee3 and Kai Ming Chan1
1 Department of Orthopaedics & Traumatology, 2 Department of Sports Science and Physical Education, 3 Lee Hysan Clinical Research Laboratory, The Chinese University of Hong Kong, Hong Kong, People’s Republic of China

Address correspondence to Ming Ju Liu, Ph.D., 5/F Clinical Science Building, Department of Orthopaedics and Traumatology, Chinese University of Hong Kong, Prince of Wales Hospital, Shantin, New Territories, Hong Kong, People’s Republic of China; tel 852 26 32 3311; fax 852 26 46 3020; e-mail liumingju{at}ort.cuhk.edu.hk.

It has been shown that many diseases are linked to abnormalities of the erythrocyte membrane. This study observed the changes in erythrocyte membrane fluidity during an immobilization period of 21 days. The right hindlimbs of male adult New Zealand white rabbits were immobilized for 21 days. Blood samples were collected and heparinized prior to immobilization and on days 1, 3, 7, 14, and 21 of immobilization. The membrane fluidity of erythrocytes was measured by spin-label electron spin resonance (ESR). The membrane fluidity was expressed by the value of order parameter (S). The results showed a significant increase of S on days 7 and 14 of immobilization (p <0.01). The highest value of S was found on day 7 of immobilization; thereafter, S gradually declined. Compared with the value measured before immobilization, there was no significant difference in the value of S on day 21. The results show that immobilization significantly affected erythrocyte membrane fluidity, indicating a systemic and temporal response of the immobilized body. This study is the first to document the time-course of changes in erythrocyte membrane fluidity in rabbits with single hindlimb immobilization during 21 days. These results may be helpful in studying the pathophysiology of immobilization.

Keywords: erythrocyte membrane fluidity, electron spin resonance, immobilization, oxidative stress






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