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Intraoperative Monitoring of Parathyroid Hormone with a Rapid Automated Assay that is Commercially Available

Address correspondence to Pai C. Kao, Ph.D., Emeritus Office, Plummer N10, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA;e-mail: kao.pai{at}mayo.edu.

The Mayo Clinic was one of the first institutions to develop an in-house rapid parathyroid hormone (PTH) assay that used homegrown antibodies to monitor plasma PTH levels during parathyroidectomy. This PTH assay is economical, but it requires highly trained technologists and an experienced laboratory director and it is difficult to perform in the operating suite. We sought a fully automated multipurpose autoanalyzer with bar code reader to identify different patients’ names and capacity to manage specimens from several patients who are having simultaneous operations. In addition, after complete tumor removal, the plasma PTH level should decrease to <25% of the baseline level; otherwise, it may indicate that the antibodies used in the assay have cross-reacted with long half-life fragments other than intact PTH, which has a half-life of only ~2 min. An automated multipurpose analyzer, the Immulite 1000 with a Turbo PTH kit (DPC), fits these criteria and has replaced our in-house rapid assay. Of 47 patients who had parathyroidectomy for primary hyperparathyroidism and were tested with the new equipment, 45 (96%) had their plasma PTH levels decrease to <25% of the baseline levels. In 41 of 47 patients (87%), the PTH value decreased to <5 pmol/L (provisional reference range) within 20 min after tumor excision. The usefulness of the PTH assay extends from the traditional diagnosis of parathyroid disease to intraoperative monitoring, helping to ensure a higher cure rate.

Keywords: parathormone, parathyroidectomy, intraoperative monitoring

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