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Annals of Clinical & Laboratory Science 32:55-60 (2002)
© 2002 Association of Clinical Scientists

Quantitative Analysis of Urine Sediment Using Newly Designed Centrifuge Tubes

Yonggoo Kim1, Dong Chan Jin2, Eun Jung Lee1, Duck Hyun Lee1, Hyun Hee Chung1, Myungshin Kim1, Jihyang Lim1, Ahwon Lee1, Kyo Young Lee1, Chang Suk Kang1, Kyungja Han1 and Soo Hwan Pai3
1 Department of Clinical Pathology, College of Medicine, Catholic University of Korea, Seoul, Korea
2 Department of Internal Medicine, College of Medicine, Catholic University of Korea, Seoul, Korea
3 Department of Clinical Pathology, College of Medicine, Inha University, Inchon, Korea

Address correspondence to Kyungja Han, M.D., Department of Clinical Pathology, St. Mary’s Hospital, College of Medicine, Catholic University of Korea, #62, Youido-dong, Young-deungpo-gu, Seoul, (South) Korea 150-713; tel 822 3779 1310; fax 822 783 6648; e-mail hankja{at}cmc.cuk.ac.kr.

We quantified the formed elements of urine sediment using newly designed plastic centrifuge tubes with top and bottom openings and a 0.5 ml sized bottom ball (YZ tube). This design minimizes the adherence of formed elements that occurs on the glass surface of conventional tubes. The numbers of white blood cells (WBC) and red blood cells (RBC) using glass tubes did not differ from those observed using YZ tubes. However, the YZ tube method detected renal casts more frequently than the conventional glass tube method; the detection rate for renal casts in normal urine samples was 21.4% vs 2.9%, in samples from hospitalized patients it was 47.5% vs 10.2%, and from patients with kidney disease it was 88.9% vs. 44.4%. Especially, the YZ tube method detected more hyaline casts in all types of samples. The correlation between the glass tube and YZ tube methods was good for WBC (r=0.996), RBC (r=0.964), and epithelial cell count (r=0.939), but the correlation was weak for casts (r=0.511 for hyaline casts; r=0.359 for other casts). In conclusion, the YZ tube method of urine sediment analyses is an easy and accurate quantitative method; it is recommended as the method of choice for detecting and quantifying pathological casts in urine.

Keywords: urine sediment, urinalysis, renal casts, YZ centrifuge tube




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[Abstract] [Full Text] [PDF]


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