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Annals of Clinical & Laboratory Science 31:75-84 (2001)
© 2001 Association of Clinical Scientists

Expression and Secretion of RANTES by Human Peripheral Blood CD4+ Cells are Dependent on the Presence of Monocytes

Pio Conti1, Renato C. Barbacane1, Claudio Feliciani2 and Marcella Reale1
Department of Oncology and Neurosciences, University of Chieti School of Medicine, Chieti, Italy 1 Immunology Division; 2 Dermatology Section

Address correspondence to Professor Pio Conti, Immunology Division, University of Chieti School of Medicine, Via dei Vestini, 66013 Chieti, Italy; tel 39 0871 3555293; fax 39 0871 561635; e-mail pconti{at}unich.it.

The cDNA for RANTES (an acronym for "Regulated upon Activation, Normal T cell Expressed and Secreted") was initially discovered by subtractive hybridization as a T cell–specific sequence. Consistent with it being a C-C (ß) chemokine, RANTES is a monocyte chemoattractant. In addition, RANTES can chemoattract unstimulated CD4+/CD45RO+ memory T cells and stimulated CD4+ and CD8+ T cells with the naive and memory phenotypes in immunologically active sites. It has been shown that CD8+ cells are dominant sources of RANTES. Here we attempted to determine if CD4+ cells express and secrete RANTES alone, or if other accessory cells (activated monocytes) are needed to activate them. We found that when autologous monocytes are added to CD4+ cells and then stimulated with phytohaemagglutinin (PHA), the quantity of RANTES, in terms of transcription and translation of the protein, is significantly higher than the amount produced by the PHA–activated monocytes alone; isolated CD4+ cells stimulated with PHA do not produce any appreciable quantity of RANTES. When CD4+ cells are primed overnight with monocytes and then stimulated with PHA, they produce more RANTES compared to PHA–stimulated CD4+ cells alone. The influence that monocytes have on CD4+ cells to produce RANTES was confirmed when the physiological activator, tumor necrosis factor- {alpha} (TNF-{alpha}), was used. These data show that CD4+ cells need monocytes to express and secrete appreciable amounts of RANTES.

Keywords: RANTES, CD4+ cells, monocytes, macrophages, dexamethasone






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