Annals of Clinical and Laboratory Science, Vol 26, Issue 1, 71-75
Copyright © 1996 by Association of Clinical Scientists
Hemoglobin interference with urinary Bence Jones protein analysis on electrophoresis
GA Hobbs
and
SS Levinson
Two cases are described which show that on urine protein electrophoresis a paraprotein owing to hemoglobin cannot be distinguished from a Bence Jones protein. A simple method is described for confirming the presence of hemoglobin: a densitometric scan of the electrophoretogram at 415 nm, in which hemoglobin absorbs in a band coincident with the restriction. Furthermore, it is shown that other serum proteins, elevated levels of bilirubin, and Bence Jones protein do not interfere with this detection system.