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Annals of Clinical and Laboratory Science, Vol 24, Issue 5, 401-406
Copyright © 1994 by Association of Clinical Scientists

Articles

Rapid quantitation of hemoglobin S by isoelectric focusing

MN Reddy and RA Franciosi

The feasibility of isoelectric focusing (IEF) to determine hemoglobin S (HbS) at a faster turn-around-time and to resolve the HbS and hemoglobin A (HbA) in presence of high concentrations of hemoglobin F (HbF) is evaluated. The IEF procedure is faster, and the results can be obtained in less than 45 minutes. The resulting data are comparable to gel electrophoresis. It is a superior procedure in resolving both HbS and HbA in the presence of high HbF and, therefore, a desirable technique to use for infants and children. Further, IEF is simpler than the gel electrophoresis, relatively inexpensive, easily adaptable for routine use, and suitable for "stat" conditions.




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Copyright © 1994 by the Association of Clinical Scientists.