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Annals of Clinical and Laboratory Science, Vol 15, Issue 3, 241-245
Copyright © 1985 by Association of Clinical Scientists

Articles

Measurement of serum chorionic gonadotropin by a biotin-avidin labeled enzyme immunoassay

E Fehskens and MW Burgett

A biotin-avidin enzyme immunoassay (EIA) for the measurement of human chorionic gonadotropin (hCG) in serum is described. This procedure involves the use of specific antibody immobilized on beads, biotin-labeled specific antibody, and enzyme-labeled avidin. Reproducible results were achieved within three hours for hCG in serum in the range of two mIU/ml to 150 mIU/ml. HCG levels as low as 0.4 mIU/ml can be measured. The biotin-avidin EIA and two commercially-available radioimmunoassay (RIA) kits were used to determine serum hCG levels on a group of patient samples. Good agreement was found between the biotin-avidin EIA and the RIA methods.




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Copyright © 1985 by the Association of Clinical Scientists.