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Annals of Clinical and Laboratory Science, Vol 11, Issue 2, 115-118
Copyright © 1981 by Association of Clinical Scientists

Articles

An improved method for concurrent isolation of human granulocytes and lymphocytes. Its application in isoelectric focusing electrophoresis

KC Poon, PI Liu, JJ Chen, CC Hong, and RC Allen

A modified method of Boyum's technique to isolate granulocytes and lymphocytes from human blood is described. The granulocytes and the lymphocytes separated by this isolation procedure are highly pure, viable, and in sufficient number for subsequent isoelectric focusing studies. Different proteins have been separated by isoelectric focusing. This technique can be useful in the investigation of granulocytic and lymphocytic disorders.




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Copyright © 1981 by the Association of Clinical Scientists.