ACLS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shipe JR,
Right arrow Articles by Savory, J
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shipe JR, , Jr
Right arrow Articles by Savory, J
Annals of Clinical and Laboratory Science, Vol 10, Issue 2, 128-136
Copyright © 1980 by Association of Clinical Scientists

Articles

High performance liquid chromatographic separation and fluorescence detection of polyamines in plasma and erythrocytes

Shipe JR Jr and J Savory

A high performance liquid chromatography has been adapted to the measurement of putrescine, spermidine and spermine in plasma and erythrocytes. Detection was by fluorimetry using o-phthalaldehyde which formed fluorophores with the polyamines. Sensitivity of the method was better than 10 pmol of each polyamine, and recovery averaged 93.9 percent. The precision of the analysis was two percent (R.S.D.). Good correlation was obtained with a reference chemical ionization gas chromatographic-mass spectrometric procedure. Reference intervals for polyamines in both plasma and erythrocytes were estimated on the basis of 25 normal volunteers.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1980 by the Association of Clinical Scientists.